In-vitro evaluation of anti-inflammatory properties of ethanol extract of ten locally obtained plants in Uyo, Nigeria
Keywords:
haemolysisAbstract
Background: Many diseases are associated with inflammatory processes. To prevent or manage these diseases, antiinflammatory therapies are required. The current anti-inflammatory drugs often result in therapy failure or precipitate intolerable adverse effects; hence, there is a need for new and more effective anti-inflammatory agents. The anti inflammatory activity of methanol extracts of ten locally obtained plants namely: A. conyzoides ; A. indica, M. oleifera, vera, T. fruticosum, Z. officinale, O. gratissimum, V. amygdalina, C. papaya, and A. sativum, were studied.
Methods: The in-vitro anti-inflammatory activity of the plants’ extracts was studied using albumin denaturation assay and membrane stabilization assay against aspirin and diclofenac sodium as standard drugs.
Results: The results showed a dose dependent increase in anti-inflammatory activity in both models. In protein denaturation inhibition, aspirin, showed maximum inhibition of 67% at a concentration of 100 µg/mL, while the extracts’ maximum inhibition was observed at a concentration of 400 µg/ml, with A. indica, and M. oleifera having the highest inhibition at 61% and 64% compared to aspirin, which showed 70% protection. For hypotonicity induced haemolysis, the maximum inhibition was observed at the concentration of 400 µg/ml, with A. indica and M. oleifera, having 70 respectively compared to diclofenac sodium, which showed 53% protection at 100 µg/mL.
Conclusion: These results suggest that these plants are potential sources of leads for the development of better and more effective anti-inflammatory agents.
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