In-vitro evaluation of anti-inflammatory properties of ethanol extract of ten locally obtained plants in Uyo, Nigeria


  • Ekarika C. Johnson Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
  • Inyene V. James Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria




Background: Many diseases are associated with inflammatory processes. To prevent or manage these diseases, antiinflammatory therapies are required. The current anti-inflammatory drugs often result in therapy failure or precipitate intolerable adverse effects; hence, there is a need for new and more effective anti-inflammatory agents. The anti inflammatory activity of methanol extracts of ten locally obtained plants namely: A. conyzoides ; A. indica, M. oleifera, vera, T. fruticosum, Z. officinale, O. gratissimum, V. amygdalina, C. papaya, and A. sativum, were studied.

Methods: The in-vitro anti-inflammatory activity of the plants’ extracts was studied using albumin denaturation assay and membrane stabilization assay against aspirin and diclofenac sodium as standard drugs.

Results: The results showed a dose dependent increase in anti-inflammatory activity in both models. In protein denaturation inhibition, aspirin, showed maximum inhibition of 67% at a concentration of 100 µg/mL, while the extracts’ maximum inhibition was observed at a concentration of 400 µg/ml, with A. indica, and M. oleifera having the highest inhibition at 61% and 64% compared to aspirin, which showed 70% protection. For hypotonicity induced haemolysis, the maximum inhibition was observed at the concentration of 400 µg/ml, with A. indica and M. oleifera, having 70 respectively compared to diclofenac sodium, which showed 53% protection at 100 µg/mL.

Conclusion: These results suggest that these plants are potential sources of leads for the development of better and more effective anti-inflammatory agents.


Nathan, C. (2002). Points of control in inflammation. Nature, 420(6917), 846–852.

Askenase, M., & Sansing, L. (2016). Stages of the Inflammatory Response in Pathology and Tissue Repair after Intracerebral Hemorrhage. Seminars in Neurology, 36(03), 288–297.

Medzhitov, R. (2008). Origin and physiological roles of inflammation. Nature, 454(7203), 428–435.

Murakami, M., & Hirano, T. (2012). The molecular mechanisms of chronic inflammation development. Frontiers in Immunology, 3(323).

Kumar, V., Abbas, A., & Aster, J. (2020). Robbin & Cotran pathologic basis of disease (10th Ed.). Elsevier.

Silva, L. M. C. M., Lima, V., Holanda, M. L., Pinheiro, P. G., Rodrigues, J. A. G., Lima, M. E. P., Benevides, N. M. B. (2010). Antinociceptive and Anti-inflammatory Activities of Lectin from Marine Red Alga Pterocladiella capillacea. Biological and Pharmaceutical Bulletin, 33(5), 830–835.

Brune, & Patrignani, P. (2015). New insights into the use of currently available non-steroidal antiinflammatory drugs. Journal of Pain Research, 105.

Aswad, M., Rayan, M., Abu-Lafi, S., Falah, M., Raiyn, J., Abdallah, Z., & Rayan, A. (2017). Nature is the best source of anti-inflammatory drugs: indexing natural products for their anti-inflammatory bioactivity. Inflammation Research, 67(1), 67–75.

Dutartre, P. (2016). Inflammasomes and Natural Ingredients towards New Anti-Inflammatory Agents. Molecules, 21(11), 1492.

Pearson, H., Fleming, T., Chhoun, P., Tuot, S., Brody, C., & Yi, S. (2018). Prevalence of and factors associated with utilization of herbal medicines among outpatients in primary health centers in Cambodia. BMC Complementary and Alternative Medicine, 18(1).

Miller, J. S. (2001). The Global Importance of Plants as Sources of Medicines and the Future Potential of Chinese Plants. Drug Discovery and Traditional Chinese Medicine, 33–42.

Venkatesha, S. H., Acharya, B., & Moudgil, K. D. (2017). Natural Products as Source of Anti-Inflammatory Drugs. Inflammation - From Molecular and Cellular Mechanisms to the Clinic, 1661–1690.

Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in West Africa. Spectrum books limited, Ibadan, Nigeria. pp: 35-44.

Trease G.E., Evans, W.C. (1996). A Textbook of Pharmacognosy (13th ed.) Alden Press, Oxford, pp: 213-232.

Leelaprakash G, S. Mohan Dass “Invitro Anti-Inflammatory activity of Methanol extract of Enicostemma axillare”, Int. J. Drug Dev. & Res., July-Sept 2011, 3(3): 189-196

Latha, S., Fatima Grace, X., Shanthi, S., Chamundeeswari, D., Seethalakshmi, S., & Uma Maheswara Reddy, C. (2011). In vitro antioxidant and anti-inflammatory activity of methanol extract of Stereospermum colais(buch.-ham. ex.dillw). Sri Ramachandra Journal of Medicine, 4(1), 11–14.

Pandey, A. K., Kashyap, P. P., & Kaur, C. D. (2017). Anti-inflammatory activity of novel Schiff bases by in vitro models. Bangladesh Journal of Pharmacology, 12(1), 41.

Chopade, A. R., Somade, P. M., & Sayyad, F. J. (2012). Membrane Stabilizing Activity and Protein Denaturation: A Possible Mechanism of Action for the Anti-Inflammatory Activity of Phyllanthus amarus. Journal of Krishna Institute of Medical Sciences University, 1(1), 67–72.

Ge, W., Li, D., Gao, Y., & Cao, X. (2014). The Roles of Lysosomes in Inflammation and Autoimmune Diseases. International Reviews of Immunology, 34(5), 415–431.




How to Cite

Johnson, E. C., & James, I. V. (2022). In-vitro evaluation of anti-inflammatory properties of ethanol extract of ten locally obtained plants in Uyo, Nigeria. Journal of Drug Discovery and Research, 1(2), 26–32. Retrieved from