In-vitro evaluation of anti-inflammatory properties of ethanol extract of ten locally obtained plants in Uyo, Nigeria

Ekarika C. Johnson; Inyene V. James

Authors

Ekarika C. Johnson Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
Inyene V. James Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria

Keywords:

haemolysis

Abstract

Background: Many diseases are associated with inflammatory processes. To prevent or manage these diseases, antiinflammatory therapies are required. The current anti-inflammatory drugs often result in therapy failure or precipitate intolerable adverse effects; hence, there is a need for new and more effective anti-inflammatory agents. The anti inflammatory activity of methanol extracts of ten locally obtained plants namely: A. conyzoides ; A. indica, M. oleifera, vera, T. fruticosum, Z. officinale, O. gratissimum, V. amygdalina, C. papaya, and A. sativum, were studied.

Methods: The in-vitro anti-inflammatory activity of the plants’ extracts was studied using albumin denaturation assay and membrane stabilization assay against aspirin and diclofenac sodium as standard drugs.

Results: The results showed a dose dependent increase in anti-inflammatory activity in both models. In protein denaturation inhibition, aspirin, showed maximum inhibition of 67% at a concentration of 100 µg/mL, while the extracts’ maximum inhibition was observed at a concentration of 400 µg/ml, with A. indica, and M. oleifera having the highest inhibition at 61% and 64% compared to aspirin, which showed 70% protection. For hypotonicity induced haemolysis, the maximum inhibition was observed at the concentration of 400 µg/ml, with A. indica and M. oleifera, having 70 respectively compared to diclofenac sodium, which showed 53% protection at 100 µg/mL.

Conclusion: These results suggest that these plants are potential sources of leads for the development of better and more effective anti-inflammatory agents.

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