Evaluation of inhibitory Effect of Solenostemon monostachyus on alpha amylase and alpha glucosidase enzymes of rats

Authors

  • Jude E. Okokon Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria.
  • Martins O. Anagboso Department of Microbiology, Madonna University Nigeria, Elele Campus, Rivers State, Nigeria.
  • Emmanuel I. Etim Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria.
  • Lynda O. Asor Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University Nigeria, Elele Campus, Rivers State, Nigeria.

Keywords:

diabetes, alpha glucosidase, alpha amylase, Solenostemon monostachyus

Abstract

Background: Solenostemon monostachyus (P. Beauv.) Brig. (Lamiaceae) a medicinal plant used traditionally in the treatment of diseases including diabetes was evaluated for effect on alpha amylase and alpha glucosidase enzymes in vivo.

Methods: The leaf extract (75, 150, 225 mg/kg) of Solenostemon monostachyus were investigated in vivo for inhibitory effect on alpha amylase and alpha glucosidase enzymes using starch, sucrose and maltose as substrates. Acarbose was used as reference drug.

Results: The leaf extract caused significant (p<0.05) and non-dose-dependent reduction in blood glucose levels of treated rats with the various substrates used.

Conclusion: The results suggest that the leaf extract of Solenostemon monostachyus have the potentials to inhibit alpha amylase and glucosidase in rats.

         Abtract Views | PDF Download: 241 / 173

References

Mve-Mba CE, Menut C, Lamaty G, Zollo PHA, Tchoumbougnang. (1994). Aromatic plants of tropical central Africa. Part XIX. Volatile components from leaves of two lamiaceae from Cameroon: Leucas deflexa Hook. and Solenostemon monostachyus (P.Beauv.) Briq. Flav. Fragr. J. 9(6):315-317.

Adebayo JO, Krettli AU. (2011). Potential antimalarials from Nigerian plants: A review. J. Ethnopharmacol. 133: 289–302.

Ajibesin KK, Ekpo BA, Bala DN, Essien EE, Adesanya SA. (2008). Ethnobotanical survey of Akwa Ibom State of Nigeria. J. Ethnopharmacol. 115: 387–408.

Toshio M, Peter R, Conrad HE. (1980). Structures of six Coleons (Diterpenoids) From Solenostemon monostachyus (P. Beauv.) Briq. (Labiatae). Helvetica Chimica Acta- 63: 95-101

N’Guessan HA, Dago DCE, Mamyrbekova-Bekro JA, Békro YA. (2011). CCM d’extraits selectifs de 10 plantes utilisées dans le traitement traditionnel de l’hypertension arterielle en Côte d’Ivoire. Eur. J. Sci. Res. 66 : 575-585.

Okoko T, Ere D. (2012). Antioxidant activities of Solenostemon monostachyus leaf extract using in vitro methods. Sci. Res. Essays 7: 621-626.

Tebekeme, O. and Diepreye, E. (2012). Antioxidant Activities of Solenostemon monostachyus leaf extract using In Vitro Methods. Scientific Research and Essays 7(6); 621-626.

Fidele KZ, Andre KB, Yao DJ, Michel OA (2012). Action of hydroethanolic leaves extract of Solenostemon monostachyus (lamiaceae) on cardiovascular system of mammalians: blood pressure lowering effects. Intl. J. Pharm. Biol. Sci. 2:310-320.

Ekundayo EO, Ezeogu LI. (2006). Evaluation of antimicrobial activities of extracts of five plants used in traditional medicine in Nigeria. Intl J. Trop Med. 1: 93-96.

Amazu LU, Antia BS, Okokon JE. (2015). Antiulcer activity of Solenostemon monostachyus. The Journal of Phytopharmacology. 4(2): 97 – 101.

. Okokon , J.E., Louis, U.A., and Nwidu, L.L ( 2015). Antidiabetic and hypolipidaemic activities of Solenostemon monostachyus. Journal of Herbal Drug. 6(2): 85-92.

Edet, A., Amaechi D., Udosen, E.O., and Uboh, F.E. (2015). Evaluation of the effect of ethanol leaf extract of Solenostemon monostachyus on Blood glucose and liver Enzymes in STZ induced Diabetic rats. European Scientific Journal. 11 (12).

Okokon JE, Davis K, Azare B. (2016a). Antimalarial and antipyretic activities of Solenostemon monostachyus. Pharm. Biol. 54:648 - 653.

Okokon, J.E., Davis, K., and Nwidu, L.L (2016b). Anti-infammatory and Antinociceptive activities of Solenostemon monostachyus aerial part extract in Mice. Avicenna Journal of Phytomedicine. 6(3); 284-294.

.Okokon JE, Oguamanam W, Umoh EE. (2017). Hepatoprotective and nephroprotective activities of Solenostemon monostachyus leaf extract. Afr J Pharmacol Therapeut. 6(2):123 - 133.

Okokon JE, Davies K., Bassey AL. (2020). Psychopharmacological study of leaf extract of Solenostemon monostachyus. Nigerian Journal of Pharmaceutical and Applied Sciences Research. 9(3): 59-63

Gidado A, Watafua M, Sa’ad RS, Tagi HS, Abdullahi S. (2019). Alpha-amylase, maltase and sucrase inhibitions by aqueous leaf extracts of Anacardium occidentale (Anacardiacea) and Piliostigma reticulatum (Caesalpiniaceae) in rats. Trop J Nat Prod Res, 3(6):210-215.

World Health Organization. Glucose analyser. Core medical equipment information. 2011. www.who.int/medical_devices/en/index.html. Assessed on 3rd June, 2019.

Kalra S. (2014). Alpha Glucosidase Inhibitors. The J Pak Med Assoc. 64(4):474-476.

Alongi M and Anese M. (2018). Effect of Coffee Roasting on in vitro α-glucosidase activity: inhibition and Mechanism of Action. Food Res Intl. 111:480-487.

Ibrahim MA., Koorbanally N.A, Islam S. (2014). Antioxidative activity and inhibition of key enzymes linked to Type 2 diabetes (α-glucosidase and α-amylase) by Khaya senegalensis. Acta Pharm. 64:311-324.

Esimone CO, Okonta JM, Ezugwu CO.(2001). Blood sugar lowering effect of Anacardum occidentale leaf extract in experimental rabbit model. J Nat Remed. 1:60–63.

Datte JY, Kpahe F, Offoumou AM. (2010). Acute toxicity and antioxidant activity of hydroethanolic extract of Solenostemon monostachyus P. Beauv. Leaves. J. Compl. Integr. Med. 7: 11-16.

Proença, C., Freitas, M., Ribeiro, D., Oliveira, E., Sousa, J., Tomé, S. M., Ramos, M. J., Silva, A., Fernandes, P. A., Fernandes, E. (2017). α-Glucosidase inhibition by flavonoids: an in vitro and in silico structure-activity relationship study. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1):1216–1228.

Su J, Tang Z. (2019). Effects of (-)-epigallocatechin gallate and quercetin on the activity and structure of α-amylase. Tropical Journal of Pharmaceutical Research, 18(3): 585-590.

Proença, C.; Ribeiro, D.; Freitas, M.; Fernandes, E. (2021). Flavonoids as Potential Agents in the Management of Type 2 Diabetes through the Modulation of alpha-Amylase and alpha-Glucosidase Activity: A Review. Crit. Rev. Food Sci. Nutr. 1–71.

Oboh G, Olasehinde TA, Ademosun AO. (2017). Inhibition of enzymes linked to type-2 diabetes and hypertension by essential oils from peels of orange and lemon. International Journal of Food Properties, 20: sup1, S586-S594.

Zhao, D. G., Zhou, A. Y., Du, Z., Zhang, Y., Zhang, K., Ma, Y. Y. (2015). Coumarins with α-glucosidase and α-amylase inhibitory activities from the flower of Edgeworthia gardneri. Fitoterapia, 107, 122–127.

Karakaya, S., Gözcü, S., Güvenalp, Z., Özbek, H., Yuca, H., Dursunoğlu, B., Kazaz, C., Kılıç, C. S. (2018). The α-amylase and α-glucosidase inhibitory activities of the dichloromethane extracts and constituents of Ferulago bracteata roots. Pharmaceutical Biology, 56(1), 18–24.

Ishnava KB and Metisariya DM. (2018). In vitro study on α-amylase inhibitory activity of selected ethnobotanical plant extra its and its herbal formulations. Intl J. Pharmacog. Chinese Med.2(3):000136.

Shirwaikar A, Rajendran K, Punitha ISR. (2005). Antidiabetic activity of alcoholic stem extract of Coscinium fenestratum in streptozotocin nicotinamide induced Type 2 diabetic rats. J Ethnopharmacol. 93:369-374.

Ortiz-Andrade RR, García-Jiménez S, Castillo-Espaňa P, Ramírez-Avíila G, Villalobos-Molina R, Estrada-Soto S. (2007). Alpha-glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana: An anti-hyperglycemic agent. J Ethnopharmacol. 109:48-53.

Yoshikawa M, Murakami T, Yashiro K, Matsuda H. (1998). Kotalanol, a potent alpha-glucosidase inhibitor with thiosugar sulfonium sulfate structure from antidiabetic agurvedic medicine Salacia reticulata. Chem Pharm Bull (Tokyo). 46:1339-1340.

Kalita D, Holm DG, LaBarbera DV, Petrash JM, Jayanty SS. (2018). Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds. PLoS ONE. 13(1):e0191025.

Funke I and Melzig MF. (2005). Effect of different phenolic compounds on a-amylase activity: screening by microplate-reader based kinetic assay. Pharmazie. 60(10):796–797.

Downloads

Published

2023-02-17

How to Cite

Okokon, J. E., Anagboso, M. O., Etim, E. I., & Asor, L. O. (2023). Evaluation of inhibitory Effect of Solenostemon monostachyus on alpha amylase and alpha glucosidase enzymes of rats. Journal of Drug Discovery and Research, 1(2), 1–7. Retrieved from https://ddrg.net/index.php/ddrg/article/view/22